DESI–MS as a tool for direct lipid analysis in cultured cells

نویسندگان

  • Anna Bodzon-Kulakowska
  • Tomasz Cichon
  • Agnieszka Golec
  • Anna Drabik
  • Joanna Ner
  • Piotr Suder
چکیده

Desorption electrospray ionization may be used as a fast and convenient method for analysis and identification of lipids in the cell culture. Oxidative stress, which usually involves changes in lipids, was used as a model of pathology to show the utility of this analysis methodology. This paper addresses the surface preparation of cell culture slides, induction of oxidative stress, and cell monolayer culture preparation as well as optimization of the analysis. Advantages and drawbacks of the method were also discussed.

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عنوان ژورنال:

دوره 67  شماره 

صفحات  -

تاریخ انتشار 2015